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Posts Tagged ‘fluorescent’

Have you ever wondered how doctors and scientists seem to know exactly how cell divides, what they look like, and what they do? At some point in your life, you may have peeked under a microscope in a biology class. You probably felt the images weren’t that interesting or colorful. But if you had done the looking through a fluorescent microscope, you would have whistled a a different tune. Why?

Light and Colors

Contrary to the common field microscope that uses reflection and absorption techniques to create magnified images of specimen, the fluorescent microscope uses light to excite specimens to emit light of longer wavelength. Fluorescence is an intrinsic property of substances where it becomes luminescent when excited by a radiation. Simply put, a fluorescent microscope is a light microscope with extended capabilities and added features. A more intense light is used in microscopy that excites fluorescence in the specimen which then emits a longer light wave length. Scientists use markers to distinguish emitted wavelengths by different colors. This technology shows digitally clear color images of microscopic organisms under probe. This technique of using transmitted light through a specimen is known as Kohler illumination, after the brilliant mind who sought to overcome the limitations of previous technologies, August Kohler.

Fluorescent Microscope in Life Sciences

Unlike metallurgical microscopes used for inspecting ceramics, metals and other inorganic materials, the fluorescence microscope finds its best uses in biology and life sciences. Rapidly expanding observation technique in medicine and biology, a range of more sophisticated techniques has evolved from it. More advanced technologies such as the multiphoton and canfocal microscopies are now combined with chromophore and flourophore advances now make intracellular observations even in unicellular molecules possible. Where the cell was acknowledged to be the smallest biological unit a few decades past, components of the human DNA are no distinguishable observations under these powerful tools.

Some have an inverted frame most suitable for viewing tissue cultures and similar applications. These designs provide illumination using an episcopic optical pathway.

Examples of Fluorescence Microscopes

Olympus BX51 Upright Microscope is a modern design of an epi-flourescent microscope with a vertical illuminator. The illuminator houses a xenon or mercury arc lamp and a turret of filter cubes. Source light travels through the lamp house through two diaphragms and into the cube holding the excitation and emission filters, as well as a dichroic mirror

Olympus IX70 Inverted Microscope. This inverted frame uses epi-illumination from an internal lamphouse. Light travels from the lamphouse via a collector lens into a cube holding the filters and a dichroic mirror

Both these examples are professional or research grade equipment. These both show the full range of capabilities a basic illuminating microscope is capable of. There are even more powerful microscopes with far more advanced features using highly advanced techniques. One of the more popular ones, confocal microscopy, now offers point-scanning capabilities with the latest from Olympus, the FluoView Laser Scanning Confocal Microscopy.

Other highly advanced techniques like Multiphoton Excitation Microscopy combine multiple techniques to capture high-definition, three-dimensional, and full color images of specimens. These are the best there is in research equipment, and these will change your life from the very first instant that you use them.

CanScope – complete solution for all your microscopy needs.
Contact: 1-877-56SCOPE(72673) or info@CanScope.ca

Yes, you can see the invisible with a fluorescent microscope in toronto. Get started using one – or a metallurgical microscope – and learn more about Kohler illumination! Visit CanScope.ca today.

In the world of microscopy – the field on the use of microscopes to view specimens and objects – lighting is of utmost importance. Whatever the kind of microscope you use – a metallurgical microscope or a fluorescent microscope – you need to have the correct illumination. You need to have the ideal light not only to see the specimen in question but to see it on its “natural state” as well. You need to get an image without the unnecessary glare or “ghost images.”

In the earlier times, there were a lot of issues on sample illumination. As a result, the images seen under microscope come out as problematic, uneven, hazy, and to some extent, incorrect. All these problems are due to incorrect or poor lighting.

It was in 1893 when almost all issues of illumination were addressed. Thank goodness for the Kohler illumination! This technique was designed by August Karl Johann Valentin K”hler, a German professor. He was also an employee of the world famous Carl Zeiss Company, the leader in optical systems and engineering.

The Kohler Illumination is known for optimizing microscopic resolution simply by illuminating the field of view in an even manner. In simple terms, this means that you will get the ideal illumination if all the elements and parts of the microscope are properly aligned.

The Kohler Illumination therefore revolutionized the design of the light microscope – the type that involves diffraction, refraction and reflection. It somehow perfects the use of light in examining specimens.

Here are some of the most important “light hurdles” that the Kohler Illumination overcame:

The Filament Image

Years before the invention of the Kohler Illumination, the filament of the bulb used in lighting up the sample being examined is visible in the sample plane. Now, if you were a scientist, or a student, you wouldn’t want a distraction in your sample plane. If this was a test, and you didn’t realize that it was the filament showing up in your sample plane, you’d answer the question incorrectly.

Numerous attempts were undertaken to get rid of that filament image. First, people started using an opal bulb. Then they also tried lowering the power of the light source. This way, the image reflected by the light is not sharp enough to register itself in the sample plane as well. Of course, there’s that opal glass diffuser – to cause a certain amount of light scattering.

Still, these attempts were not perfect in eliminating the filament image. In addition, they cause even more problems. For instance, if you reduce power of the light source, you could have reduced quality of light as well. As a result, you might not be able to clearly see the specimen in your sample plate. Then there’s that question on uniformity of light.

With the Kohler Illumination, however, the light microscope saw a different kind of “light.” It was able to produce light at optimum levels. It was able to answer lighting issues – that of the filament image, most especially.

Indeed, thank goodness for the Kohler Illumination!

CanScope – complete solution for all your microscopy needs.
Contact: 1-877-56SCOPE(72673) or info@CanScope.ca

Learn how to achieve Kohler Illumination. Get the best deals of a metallurgical microscope in Toronto or a fluorescent microscope at CanScope.ca. Visit their site now!