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Archive for October, 2009

Looking at the world through your naked eyes is a treat in itself. However, looking at the life and its creations and creatures through a compound microscope is a whole different ballgame mainly because you start to gain an appreciation for life’s intricacies and subtleties in more ways than one.

As such, before splurging on something as important as a compound microscope, be sure that you factor in these compound considerations that go into buying it. These factors ought to make certain things clearer to your mind’s eye much as a microscope makes things clearer to your naked eye.

Parts

Plastic parts might be cheaper and colorful than metal parts in a microscope, yes, but you must opt for durability and quality more than beauty and quantity. As such, always choose microscopes that are made of mostly metal parts.

You will get more mileage from a metal microscope, not to mention that it ought to retain its basic beauty even after years of use. Plus, it does look more professional sitting atop your home lab desk than a toy plastic microscope!

Also, do opt for all-glass lenses. Although there are toy microscopes that promise magnifications of up to 600 times, their lenses simply will not allow for said power.

Power

A compound microscope usually comes in two basic types: low power and high power. Each type has its applications although the high power microscope is preferred for most laboratory activities.

The low power microscope magnifies visible specimens from 10X to 80X their normal sizes. Think of things like grains of sand, coins, fabric, small insects and leaves, which a budding scientist will be interested in exploring in minutiae.

The high power microscope, on the other hand, allows for magnification of 100X-1000X of invisible objects like blood cells, microorganisms and bacteria. This compound microscope, of course, requires proper training and handling.

Of course, if the microscope is intended for children, a low power type ought to suffice since it does not require elaborate specimen preparation and complex operation. When the children gets older and still have need of a microscope, then it is logical to purchase the high power type.

Price

Now, price is the last consideration because you ought to be willing to pay for a quality microscope. You don’t have to worry too much about the price though as you can purchase a good microscope for as little as $100!

If you do settle for a cheaper microscope, be sure that it fits your needs perfectly. After all, you don’t want to shell out money for a compound microscope that will not prove itself worthy of its price, no matter how small it may be. Don’t compound your money woes with virtually useless equipment, in other words.

When all these factors are considered in your purchase of the perfect compound microscope, you will discover that life, indeed, can be good despite the nitty-gritty details that we might not want to see. Think of the additional cost but, hey, when it comes to education for the whole family, nothing should be too expensive!

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Welcome the Digital Age through a digital microscope! With its eight benefits to be enjoyed, all other microscope will look small in comparison, pun intended.

Integrated Illumination

With optical microscopes, the separate illumination makes for a more complicated setup and lesser precision in reproducing the same image. Keep in mind that a small change in illumination makes for a substantial change in the image seen through the microscope.

Not so with the digital microscope! Its integrated illumination means that preliminary setup procedures are virtually eliminated. Plus, you can reproduce the same image over and over again precisely because the illumination basically remains the same.

No Target Disassembly Required

To make setup easier, you don’t have to disassemble – cut up, chop down, process – the target since it can be studied as is, thanks to the portable lens of the microscope. Basically, it’s a point-and-magnify process where you just point the lens towards the target and then magnify it according to your needs. As such, you can make quick analysis of the target!

Finer Depth of Field

Of course, optical microscopes can provide magnifications of 1000 times. However, the depth of field suffers in comparison to a digital microscope by at least 20 times! If you want finer details of the target, you just need to adjust the focus and voila! You have a clearer and deeper picture of the target.

Depth Composition and 3D Display

In addition to depth of field, you are also provided with sharper images of targets. This allows for 3D projections that represent the target conditions at the minutest detail, which is not often possible with an optical microscope limited to 2D images. Think of the detailed studies possible of the subtleties of the target!

Zoom Lens Feature

Unlike an optical microscope with its knobs to increase magnification, a digital microscope has a zoom lens feature that allows for quick and easy magnification. You don’t have to lose sight of the target while searching for the optimal magnification at which you can study it in detail.

All-Angle Study

There are times when you simply must view the target at all angles without losing focus. In digital equipment like the microscope, this is made possible through the 360-degree rotational capabilities of the digital microscope’s camera. Other features like the multi-viewer system and the origin check function, if it’s available, also provide for said versatility. Easy Share in Real Time

In an optical microscope, everybody has to take turns peering at the eyepiece just to get a similar view of the target, which is time-consuming, to say the least. With the digital type, the image of the target can be projected on a bigger screen and, hence, many people can see the same image at once. This is a great tool in a class setting.

Easy Save

Unlike an optical microscope where the process of saving the images can be complex – from microscope to media to computer – its digital counterpart allows for direct saving on its onboard hard disk drive. And the best thing is that you can organize the images directly on the microscope!

So, with all these benefits of a digital microscope, isn’t it about time that you get one fro yourself?

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Microscopes come in many forms and types. Gone are the days when the only terms related to the microscope are the electron and the compound microscopes. Today, there are numerous kinds and you don’t even know what each is really used for. You can’t even differentiate one type from another.

To clear that clutter on your mind, let’s get a few things straight.

First, the modern microscope can be classified according to compound or stereo. The compound microscope uses only one eye-piece (that cylindrical part of the microscope that you put your eyes on to see the specimen being examined). On the other hand, the stereo microscope uses two optical paths and has the ability to give you a 3D image. It is for this reason that this kind of modern microscope is very popular in surgery and in dissecting objects and even machine tools.

Second, there are two frame types of the modern microscope: the upright microscope and the inverted microscope. For you to have a clearer understanding on the difference of the two, you must be familiar with the major parts of the microscope first.

* The Illumination system – this refers to the group of parts that gives lighting to the specimen. This group includes the lamp, the condenser, the diaphragms (or pinhole apertures) and the rheostat, among others.

* The Stage – this is where the specimen rests. Usually, the specimen is held in place and moved with the use of clips and a micromanipulator, respectively.

* The Lens system- these are the group of parts responsible in forming the image. This includes the eyepiece, objective lenses, tube and the nosepiece (the mount that holds several objective lenses).

To distinguish an upright from an inverted microscope, you only need to remember the placement of the three major microscope parts mentioned above. The upright microscope is your standard view of a microscope: on top is the lens system, followed by the stage, and then the illumination system. The inverted microscope, on the other hand, has the reverse sequence. You have the illumination system on top, then the stage, followed by the lens system.

Does an inverted microscope make sense? At the outset, the idea might seem ridiculous. But upon careful consideration, you’d realize how useful an inverted microscope is.

This kind of frame is very useful in examining a specimen that’s either too large or too heavy. Yes, an inverted microscope would come in handy if you want to study cells in suspension. This is because the lenses are closer to the bottom of the specimen – where the cells are. Thus, it makes more sense to make use of an inverted microscope rather than an upright microscope in this scenario.

Microscopes – just like cameras – use accessories too. Among the widely used accessories are: the Epi-fluorescent attachment kit, a mechanical case, or even a microscope case. These make your microscope become more powerful (just when you think they could not get any more powerful, huh?).

So there you are, a little clarification about the many terms related to the microscope. To review, the modern microscope can be classified as either stereo or compound. But in relation to frame types, only two words must come to mind: upright and inverted. Easy enough, right?

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Surely, you’ve seen a microscope. You’ve had that in your elementary Science class. But did you know that there are a lot of types of microscopes on sale today?

What you’ve probably seen is one of those student’s microscopes. It is a low power optical microscope that has been marketed specifically for non-scientists – that is, to be used by students in primary and secondary school.

As mentioned, the student microscope is an optical microscope – the type that uses a system of lenses and visible light in seeing through images. This is the oldest and the simplest kind of microscope in that this still uses eye-pieces for an observer to have a glimpse of the magnified images.

On the other hand, there’s the digital microscope. It the more modern type, and more often, it does not use eye-pieces. In lieu of the eye-piece, it uses a camera and its output is a digital image projected in a computer monitor.

Using a Digital Microscope

So, how do you use a digital microscope then given that you cannot look through an eye-piece to observe a sample? Simple, think robots.

If you want to clearly see the micro-printing in a dollar bill, for example, just place the bill right below the camera of your digital microscope. The images will then be projected in your computer monitor.

But before you discover the secrets of the dollar bill, you must first install the software of your digital microscope into your computer. Your microscope package should include an installation CD, otherwise, you might be getting a fraudulent microscope.

After you’ve successfully done this, connect your digital microscope and your computer using USB cables. Find the appropriate port. You don’t have to be a computer engineer to know this. The secret to this technical task is in finding the port that fits with your cable. If it does not fit, it is not supposed to be plugged there.

When you’ve connected the correct cables and you see that such a connection is working (i.e. when you move your camera, the image on the monitor also changes), you can now get your dollar bill for scrutiny.

Adjust some knobs in your precious digital microscope until the image in your monitor becomes clear. After which, you can now start doing your digital scrutiny of the dollar bill. You can even save a captured image and do some work on it – label it, make some notes about it, or put a comment on it.

Indeed, by using a digital microscope, you are finding better answers to your questions about the world around you. You are discovering more things.

Gone are the days when you have to prick your finger, get a sample of your own blood, put it in a microscope slide and use a student microscope to observe the discs and color of your blood. Today, you have other kinds such as the digital microscope, the research microscope, and an improved version of the compound microscope.

Science indeed has transformed itself to provide us with better answers. It has even made its gadgets better to provide you with the needed tools to satisfy a more curious you.

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In 1953, the world recognized the scientific genius that is the phase contrast microscope. Forget high useful – it wasn’t just useful, it was crucial for actual live observations of intra-cellular processes like cell division.

Medical and biological sciences research are now focused on the real time, real life observation of living organisms to analyze its components and understand life better than we already do. Specifically designed for live specimens, this type of microscope enables observation of virtually invisible occurrences within a cell.

Obviously more advanced than the typical light microscope, this instrument uses the various refractive abilities of different objects to heighten the contrast between colorless and transparent structures. Under this powerful type of microscopy, specimens remain alive as they do not need to be stained – colored or dyed – to distinguish characteristics.

Light Waves and Visible Contrasts

Light waves peak and valley in regular intervals. The moment they line up, they’re said to be in phase; otherwise they’re said to be out of phase. This basic understanding of light waves is the key to how a phase contrast microscope works — it influences the optical path of light through transparent or colorless objects under observation.

Light waves passing through transparent portions of the specimen is slower then the uninfluenced light. To cause a difference in brightness, a transparent phase-plate mounted on the microscope increases the phase to half a wavelength, making the transparent object outshine its surroundings. This unique feature of phase contrast microscope makes it an indispensable tool in medical and biological observations of living cells.

How It Works

A phase contrast microscope makes use of two light sources — one under your specimen and another that is reflected off your specimen. Now, light passes through any transparent object but gets reflected off the surface of a solid, colorless object. When these light waves condense on a lens placed just above your specimen, it is easy to see if the light waves are in or out of their phase. This phase condenser lens on this type of microscope is analogous to the fluorescent filters in fluorescence microscopes; both make light differentiation possible.

Advances in Biomedical Microscopy Technologies

The latest in biomedical microscopy is the introduction of infinity-corrected phase contrast microscopes. Over the last decades, the largest manufacturers of research and/or professional grade microscopes have introduced this new type of optical system into various microscopy modules. This technology places another lens between the objective and the eyepiece and sets the object image to infinity. This correction makes it possible to introduce auxiliary components in the microscope.

This integrated capability makes imaging a an exciting part of the research. Now, you can capture, store, display — virtually do anything — with images of your specimen. With the flexibility of integrated digital technology, you can capture full color photographs or videos of your specimen and display, store and retrieve them from your computer. These capabilities produce virtually the same images on eyepieces and photo ports, allow you to hook up various types of cameras on to the microscope, which would definitely make training and demonstration more real life.

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When you have a pet, you do everything in your power to keep them safe. You do everything to keep them always healthy- never mind the amount of money that you need to spend for veterinarians and vitamins.

Sometimes, however, pet owners become paranoid that every time something’s wrong with their cat or dog, they immediately go to the vet. In reality, not all instances require you to take that trip to your vet. But better be safe than sorry, right? So here are a few situations when going to the vet is indeed mandatory:

a. Eye Trauma

When your dog is fond of sticking his head out of your car’s window, he is putting himself in more danger. There’s a very high possibility that he will catch foreign objects in his eye. This habit will make him vulnerable to flying bugs and debris.

Because you cannot really tell what it is that’s in his eye, better get a word from your vet. When needed, such an object maybe examined using veterinary microscopes. This will give your vet a closer look to what it is that’s bothering your pet.

Remember, eye trauma usually gets worse when left untreated. So even if you don’t see any immediate “complaint” from your pup, might as well be sure. Let the vet tell you what that foreign object is, let his veterinary microscopes tell you what it is.

b. Abrasions and lacerations

While abrasions are quite common among pets – when they play too much and they end up having their version of a “scraped knee” – you don’t want to risk it. Dogs can accidentally bump into your glass door or in one of your child’s educational microscopes.

True, some wounds can be cleaned using household first-aid kit, but sometimes, you need the opinion of your vet. After all, all wounds are different and some may become infected. Let your vet do his work. If he needs to use one of his veterinary microscopes to rule out infection, let him be.

c. Dog diseases

If your favorite pet is suffering from fever, loss of appetite and muscle pains, you may want to rush him to your vet. He may already be suffering from Leptospirosis or from tick fever. The vet will subject your dog to some tests – some of which using veterinary microscopes – so that he can give you the correct diagnosis and treatment.

Some dog diseases can be prevented through vaccination. Thus, it’s important that you have annual vaccination for your dog to keep him healthy and lively. Of course, having him vaccinated keeps you away from stress, from vet clinics and veterinary microscopes (and from all its too-technical-sounding parts like fluorescent filters cube), and from spending a lot of money medicating him.

Pets are a man’s best friends. If you don’t have kids, you can get pets to keep you company. Indeed, it’s just proper that you keep them in optimum heath all the time – it’s but proper that you give them the medical care that they need – aside from the tender loving care that you generously provide.

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When you purchased your first microscope, you probably thought that it contained everything you will ever need to study living and non-living things in small detail. In some ways, you are right. However, you will still need microscopy accessories as you go along in your study.

Choosing the Right Accessories

Each type of microscope requires a specific set of accessories precisely because of their unique features. Of course, all microscopes have a common thread – seeing things on a microscopic level – but the technology used, the depth of field, and the most common applications often differ.

For example, a digital microscope has a similar depth of fields as that of the slide microscope except that it allows for the creation of virtual slides and the sharing of information. Or the difference between a slide microscope and a stereo microscope makes for relatively unique sets of microscopy accessories.

Thus, before purchasing accessories for your microscope, read the instruction manual, determine the specific part that you need and assess where you can buy them. Keep in mind that branded microscopes often don’t have interchangeable interior parts since product integrity must be maintained. Unless, of course, you are buying highly interchangeable exterior microscopy accessories like slides and dust covers, then it’s another story altogether.

It’s always best to approach the authorized vendor of a specific part. This way, you are assured that it is of high-quality, hence, will contribute to longer microscope life. And always check that the part you have is exactly what you need!

Now, when it is time to install the microscopy accessories, you can either do it on your own or call the help of a professional. The latter is advisable for interior parts as these can be fragile and highly technical. For exterior parts like stage clips and slides, you might as well do it yourself.

Replace with New Microscope or Replace Old Parts

Microscopes can be expensive with prices running into the hundreds of dollars for top-of-the-line units. Thus, its parts and accessories can be on the high-end, too. Still, if you take good care of your microscope, it might be a long time yet before you have to search for microscopy accessories in terms of replacement parts.

But what happens when you have to replace old parts and the replacement costs is greater than the price for a brand new unit? Well, you either go for practicality or for sentimentality. Yes, some people can get attached to their microscopes, even baptizing them with names, so it will be quite understood if you opt for sentimentality.

If you opt for practicality, then good for you! This is because you might have to spend more money on future repairs for an oft-repaired microscope. Think of it as a rundown car that has become friends with the mechanic and enemies with your pockets.

In conclusion, there is no shame in being a dummy when it comes to microscopy accessories. After all, you cannot be expected to be a genius with microscopes on your first try!

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You have seen many Biology books to know that the microscope have helped us catch a glimpse of organisms, cellular matters, and parts of our body that cannot be seen by the naked eye. The microscope, after all, can magnify objects through the use of specialized lens and light. But what about objects that are transparent? How have scientists have come up with images of organisms that do not absorb light? How about those that are naturally colorless? The answer lies in a microscope called phase contrast.

Introduction to Phase Contrast Microscopy

Our eyes can only see colors of the visible spectrum and the differing intensities of light. Objects that absorb light are easy for us to observe because of these biological capability. Even if the objects are very tiny, it’s still possible for us to see them if we use a microscope. However, transparent and colorless objects, such as bacteria, sperm tails, flagella, and some parts of the cell, cannot be seen clearly under typical light microscopes. This is because light travels through these objects in a way that our naked eye cannot detect. Light passes through these specimens, called phase objects, slower and they are shifted. This change in phase cannot be detected by our eyes. This is why it would be impossible to study these objects clearly.

In the 1930s, a Dutch scientist named Frits Zernike developed the phase contrast method. He observed that it is possible to increase change in phase or shift in these transparent objects by half a wavelength. This was done by the use of rings etched onto plates of glass. The method resulted in patterns of interference. These patterns, in turn, made the details of the phase objects darker than the background. The contrast is increased and they become visible to the naked eye.

Phase Contrast Microscopy Today

Zernike received a Nobel price for inventing the method. It was a well-deserved accolade because he has revolutionized the way microscopy works today. Because of his invention, we have been able to properly observe objects that would otherwise have been impossible to analyze under a normal light microscope.

If it weren’t for this method, for example, we would never have known how cell division works. Without phase contrast microscopes, transparent and colorless objects are stained so that they can be observed under the microscope. This staining method makes them absorb color but it alters their components. It can kill some phase objects, too. Incidentally, killing phase objects also makes them more visible but it becomes impossible to observe their processes. Killing them often defeats the purpose of observation. With this technique, it is possible to observe living cells and how they divide.

Used with other modern devices, this kind of microscope has even made it possible to see the internal structures of these phase objects. Post-processing and other enhancement devices can now make us see what goes on inside transparent and colorless organisms. They used to be beyond the reach of technology but a genius changed that. Science and mankind has definitely benefited from this incredible invention.

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What do you know about inverted microscopes?

Inverteds (as they are called) got their name because the recognized standard – the upright microscope – works in a specific way, and is the most easily recognized orientation of a microscope. Upright microscopes look down at specimens with light source below it. These are the types of microscopes usually used by medical technologies doing your blood work; inverted ones, on the other hand, look up the specimen with light sources above the specimen.

Although the equipment was first introduced in the 19th century, it saw use in the observation of marine life only during the first years of the 20th century. During World War II, the inverted microscope was used to analyze solid, heavy metals like iron and steel. These days, inverteds are useful in many types of research that use large, heavy or otherwise bulky samples requiring a wide berth of geometry.

Aquatic Research and Sciences

Although the ecology in deep seas can be recreated for observation of a small bowl or a miniature aquarium, the container holding the specimen requires a relatively wide staging. Additionally, aquatic live and living organisms are prone to gravity and tend to settle down the base of the container. This makes it ideal to place the sample or its container above to allow for more flexibility and to be able to conduct observations in more natural circumstances.

Modern Microscopes

By now, you’d have surmised that inverteds are useful in several fields, particularly in research observations. Besides aquatic and materials research, inverteds are also useful in observing tissue culture on petri dishes — a common method used in biological sciences and medicines research. Modern inverted microscopes however, are designed for flexibility, with the user in mind.

Today, most modern microscopes are able to multi-task, with the whole ensemble being a collection of interchangeable parts that can be fitted together to serve a purpose. Manufacturers have worked hard and are working hard to meet the demands of the various sectors and users of microscopes. They are also integrating microscopy techniques to create separable modules.

In this day and age, the more modular a microscope is, the more economical it is. Manufacturers make a wide range of modules to suit many purposes across disciplines, users, and even across different microscopy techniques. So, if you find an inverted microscope with an epi-flourescent attachment, don’t be surprised. That’s evolution at work.

Technology Integration

Besides the modularization of inverted microscopes, new designs include internal light sources that are below or parallel to the specimen. Most viewing and recording controls are still below the sample. This new design allows you to put specimens directly on top of the microscope. This, in turn, allows you to manipulate sample placement in a wide geometry of choices for total flexibility.

More advanced models integrate video capabilities for specimen scanning and direct digital or analog data recording. This is very useful in various laboratories that require fast, data analysis. Often, these video microscopes are integrated into the laboratories digital or automated systems for sample analysis.

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What do you know about the compound microscope?

Probably a common apparatus at most high school science laboratories, the compound microscope use the popular combination of dual lenses and light reflection. In a world becoming infinitesimally smaller in each generation, microscopes of all types, caliber, sizes and capabilities can be found virtually everywhere — from laboratories in high school campuses, colleges, universities, to research and development laboratories of pharmaceutical companies and a host of other manufacturers.

Getting to Know Microscopes

The compound microscope has more than one lens — usually two — to do its job. The most basic of all types — the simple microscope uses a single lens. Impliedly, it offers a better view and more capabilities. Here are basics of microscopy:

1. Light Source.

Illumination is a basic requirement in microscopy. Some microscopes have mirrors that reflect light from sources outside of the microscope. Others, like the fluorescence microscope have their own specific sources of illumination, in its case a mercury-vapor lamp or a xenon arc lamp. Those using specific light sources are generally more powerful as these lights are more intense than generic day or lamplights used by ordinary compound microscopes.

2. Objective Lens.

All microscopes have objective lens — the lens closest to the object under probe; not all microscopes have eyepieces. Although the common image the word conjures is that of an ocular device, there are highly advanced microscopes that do not have an eyepiece. Some microscopes have three eyepieces for normal viewing and for mounting a camera, like the trinocular microscope. Instead, there are mounted on cameras and or video displays for a state-of-the-art, 3D view of microscopic objects or organisms. If you follow popular American TV, you would have already seen this in shows like House, MD.

3. Magnification Controls.

Yes, the term sounds impressive but it’s also very basic. Microscopes, regardless of power and/or grade, allow you to control magnification levels with adjustment knobs. Usually, the magnification power is a factor of the objective lens and the eyepiece. In all cases, the maximum magnification is 2000x. Practical Applications

Compound microscopes are used in many fields and for various purposes. The type of compound microscope you need and the magnification levels you require is determined by what you want to see, the specimen you’re using and what you want to do with the images. Incidentally, manufacturers have developed a range of microscopes with specific applications and users in mind. It’s now common to find different grades of one type of microscope. Student-grade compound microscopes — probably an inexpensive tool — are found in virtually all high school laboratories across the globe. Professional-grade compound microscopes can be found in research laboratories where you usually find white coats. Fortunately, manufacturers of this equipment like Meji Techno, Nikon, and Olympus make customizing microscopes possible with a range of selections that come with various accessories. You can configure your own microscope to suit your specific application.

But wait! That’s not all. Working closely with its user base, these companies are constantly working toward improving their existing line with creative and imaginative input from people who use their products the most. So, have you and your compound microscope met?

CanScope – complete solution for all your microscopy needs.
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A compound microscope in Toronto can get you started on the road to discovery. Check out CanScope.ca’s selection of microscopes in Toronto, microscope accessories in Toronto , and more!